Human dynein and sperm pathology.
نویسندگان
چکیده
منابع مشابه
Human dynein and sperm pathology
Human spermatozoa with normal structure and with different axonemal deficiencies (absence of axoneme, of arms, or of central structures) were studied by electron microscopy, SDS-polyacrylamide gel electrophoresis, and ATPase activity measurements. Normal human sperm possess a complement of high molecular weight polypeptides with an electrophoretic migration similar to that of sea urchin and oth...
متن کاملBiochemical identification of dynein-ATPase activity in human sperm.
Dynein-ATPase is the intracellular motor for sperm motility. In the present work we assayed the dynein-ATPase activity in an axoneme-containing fraction of human sperm, free of plasma membranes, in normozoospermic and asthenozoospermic donors. Axoneme-containing fractions were isolated from semen samples obtained from healthy donors with either normozoospermia or asthenozoospermia, as indicated...
متن کاملPotent inhibition of dynein adenosinetriphosphatase and of the motility of cilia and sperm flagella by vanadate.
The motility of demembranated sea urchin sperm flagella and that of embryo cilia reactivated with 0.1 mM ATP are completely inhibited by 4 micron and 0.5 micron vanadium(V) [V(V), in vanadate], respectively. The Mg2+-activated ATPase activity (ATP phosphohydrolase, EC 3.6.1.3)of the latent form of dynein 1 is inhibited 50% by 0.5-1 micron V(V), while the Ca2+-activated ATPase activity is much l...
متن کاملHuman Pathology
The popularity of this text is attested by the fact that this, the 5th edition, was necessitated after a lapse of but 3 years. Certain, but by no means major, revisions have been made in this edition. The style is concise and lucid. A number of the illustrations are excellent and most of the others are good. One of its most attractive features is the inclusion of an adequate bibliography after ...
متن کاملActivation of Ciona sperm motility: phosphorylation of dynein polypeptides and effects of a tyrosine kinase inhibitor.
A high molecular mass dynein ATPase polypeptide and a 18-20 kDa dynein light chain of Ciona sperm flagella are phosphorylated during in vivo activation of motility or in vitro activation of motility by incubation with cyclic AMP. A similar level of phosphorylation of these proteins is obtained by incubation of washed, demembranated spermatozoa with catalytic subunit of cyclic AMP-dependent prot...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1981
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.88.1.102